| 1. | Codon usage in genes coding for proteins with different folding types
不同折叠类型蛋白编码基因的密码子使用 |
| 2. | Method for the design of mrna detecting genechip based on codon usage bias
一种基于密码子使用偏性的检测型基因芯片设计方法 |
| 3. | Analysis of synonymous codon usage in aeropyrum pernix k1 and other crenarchaeota microorganisms
嗜热泉生古细菌及其他泉古菌同义密码子使用偏向性分析 |
| 4. | The start reading framae and stop codons , base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods
本研究在蛋白质编码基因起始阅读框和终止密码子、蛋白质编码区的碱基组中文摘要成、氨基酸及密码子的利用等方面把少棘蜈蚣与另三种多足类进行了比较。 |
| 5. | As a typical protein - coding gene , the second positions were least variable , first positions were more variable , and third positions were the most variable . although codon usage is effected by a / t bias , the amino acid composition is not varied with this bias
密码子以a 、 t结尾频率高，第三位是g的密码子使用较少，反映出cytb基因在密码子的使用上具有偏向性，同果蝇和蜜蜂相一致。 |
| 6. | Expression of monellin gene and vitreoscilla hemoglobin gene in the methylotrophic yeast pichia pastoris according to the monellin amino acid sequence and pichia pastoris preference codon usage , the monellin gene was designed and synthesized
同时探讨vgb基因在毕赤酵母中的功能，并应用于高甜度monellin重组酵母，解决毕赤酵母高密度发酵中存在的供氧不足的问题，进一步提高高甜度monellin的产量。 |
| 7. | Conclusion : it ' s possible to improve the expression efficiency by adjusting the g + c content of tir region and codon usage . replacement the cystein 78 and cystein 96 into serines , higher bioactivity gained compared with control hbfgf protein , and decreased amout of dimer and inclusion body , this suggested that free thiol group of cys78 and cys96 is the main reason of dimer and multimer formation
结论：通过调整tir区g c含量及增加大肠杆菌偏爱的密码子，确实能提高hbfgf在大肠杆菌中的表达量，而将78和96位上的半眯氨酸替换成丝氨酸后，得到的hbfgf的蛋白活性高于未改构蛋白，二聚体和包涵体减少。 |
| 8. | Aiming at low expression of exogenous gene , t - pa gene was designed . the designed t - pa gene was used to transform cucumber and the transgenic plants was obtained . at the same time , codon usage of potato , cucuber and strawberry was analyzed and forecasted the expression of t - pa gene in 3 organisms and designed codon of t - pa gene to fit for expression in plants
针对外源基因表达量低的问题，对t - pa基因进行了重新设计，并将新设计的t - pa基因转化黄瓜，获得了转基因pcr阳性植株；同时对马铃薯、黄瓜和草莓的密码子用法进行分析，预测了t - pa基因在3种生物体中的表达情况，进行了适于植物中表达的t - pa基因密码子改造。 |
| 9. | For these goals , the fo llowing jobs have been done and some results have been obtained . 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage , vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively , the modified vgbm gene of full length of 450 base pairs was synthesized . 2 the transformants were obtained after pbv221svhb was introduced into e . coli
为此本论文作了以下工作并得出了一些结论： 1根据透明颤菌血红蛋白( vhb )的氨基酸序列，选用植物偏爱密码子，对透明颤菌血红蛋白基因( vgb )进行优化改造，设计并合成了22条寡核苷酸短片段，人工合成了改造的全长450bp的透明颤菌血红蛋白( vhb )基因( vgbm |
